Plant Transcription Factor Database
Previous version: v3.0
Spinacia oleracea
FAR1 Family
Species TF ID Description
Sp_019230_oihr.t1FAR1 family protein
Sp_026180_qgyj.t1FAR1 family protein
Sp_030330_jiiy.t1FAR1 family protein
Sp_045540_ymir.t1FAR1 family protein
Sp_070430_ceyz.t1FAR1 family protein
Sp_072010_dtqg.t1FAR1 family protein
Sp_097650_cyaa.t1FAR1 family protein
Sp_105270_kyxt.t1FAR1 family protein
Sp_105270_kyxt.t2FAR1 family protein
Sp_105270_kyxt.t3FAR1 family protein
Sp_105280_gdhp.t1FAR1 family protein
Sp_116620_jzkx.t1FAR1 family protein
Sp_144140_rucw.t1FAR1 family protein
Sp_144140_rucw.t2FAR1 family protein
Sp_155670_kaif.t1FAR1 family protein
Sp_155670_kaif.t2FAR1 family protein
Sp_157270_hzna.t1FAR1 family protein
Sp_168680_zrgt.t1FAR1 family protein
Sp_169420_jssj.t1FAR1 family protein
Sp_172870_ahnk.t1FAR1 family protein
Sp_196630_pyjn.t1FAR1 family protein
Sp_197440_axky.t1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885